LAPORAN PRAKTIKUM

MIKROBIOLOGI PERTANIAN

Disusun Oleh :

Nama : Muhammad Garda Pancasona

NIM : H0718105

Kelompok : 10

LABORATORIUM BIOLOGI DAN BIOTEKNOLOGI TANAH

PROGRAM STUDI ILMU TANAH
FAKULTAS PERTANIAN
UNIVERSITAS SEBELAS MARET SURAKARTA
2019

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 HALAMAN PENGESAHAN

Laporan Praktikum Mikrobiologi Pertanian ini di susun guna melengkapi

tugas mata kuliah Mikrobiologi Pertanian. Laporan ini telah diketahui dan disahkan
oleh Dosen dan Co Ass Biologi Tanah pada :

Disusun oleh:

Nama : Muhammad Garda Pancasona

NIM : H0718105
Kelompok : 10
Co – Assisten : Linda Pramudiya Wardani

Mengetahui,

Dosen Koordinator Praktikum Co Asisten

Mikrobiologi Pertanian

Prof.Dr.Agr.Sc.Ir. Vita Ratri Cahyani, M.P. Linda Pramudiya Wardani

NIP. 196612051990102001 NIM. H0217040

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 HALAMAN PERSETUJUAN
LAPORAN PRAKTIKUM MIKROBIOLOGI PERTANIAN
SEMESTER SEPTEMBER-DESEMBER 2019
PROGRAM STUDI ILMU TANAH

NAMA : Muhammad Garda Pancasona

NIM : H0718105
KELAS : Agroteknologi B
KELOMPOK : 10

Surakarta, Desember 2019

Menyetujui TAHAP I TAHAP II

Co-Asisten Praktikum Pemeriksa

Tanggal Tanggal
Linda Pramudiya Wardani
NIM. H0217040
Mengetahui,
Dosen Pengampu Kelas

Dr. Ir. Widyatmani Sih Dewi, M.P. Tanggal Tanggal

NIP. 196311231987032002

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 KATA PENGANTAR

Puji Syukur ke hadirat Allah SWT karena rahmat dan karunia-Nya penulis
dapat menyelesaikan laporan Praktikum Mikrobiologi Pertanianini dengan baik.
Dalam penulisan laporan Praktikum Mikrobiologi Pertanian ini tentunya tidak
lepas dari bantuan dari berbagai pihak. Untuk itu penulis ingin mengucapkan terima
kasih kepada :
1) Dekan Fakultas Pertanian UNS
2) Tim Dosen Mata Kuliah Mikrobiologi Pertanian terima kasih atas segala
bimbingannya
3) Tim CoAss Praktikum Mikrobiologi Pertanian
Penulis mohon maaf bila dalam laporan ini terdapat kata-kata yang kurang
berkenan. Harapan penulis semoga laporan ini dapat bemanfaat dan menunjang mata
kuliah Mikrobiologi Pertanian.

Surakarta, Desember 2019

Penyusun

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 DAFTAR ISI

HALAMAN JUDUL .......................................................................................... i

HALAMAN PENGESAHAN ............................................................................ ii
HALAMAN PERSETUJUAN ............................................................................ iii
KATA PENGANTAR ....................................................................................... iv
DAFTAR ISI ...................................................................................................... v
DAFTAR TABEL ............................................................................................... viii
I. PENGENALAN ALAT, BEKERJA SECARA ASEPTIK, STERILISASI
DAN PEMBUATAN MEDIA ..................................................................... 1
A. Pendahuluan ............................................................................................ 1
1. Latar Belakang .................................................................................... 1
2. Tujuan Praktikum ............................................................................... 2
B. Tinjauan Pustaka ..................................................................................... 3
C. Metodologi Praktikum............................................................................. 7
1. Waktu dan Tempat Praktikum ............................................................ 7
2. Alat ..................................................................................................... 7
3. Bahan .................................................................................................. 8
4. Cara Kerja ........................................................................................... 9
D. Hasil Pengamatan dan Pembahasan ........................................................ 11
1. Hasil Pengamatan ............................................................................... 11
2. Pembahasan ........................................................................................ 17
E. Kesimpulan dan Saran ............................................................................. 24
1. Kesimpulan ......................................................................................... 24
2. Saran ................................................................................................... 24
DAFTAR PUSTAKA

II. ANALISA MIKROBIOLOGI UNTUK BAKTERI DAN FUNGI ............. 25

A. Pendahuluan ............................................................................................ 25
1. Latar Belakang .................................................................................... 25
2. Tujuan Praktikum ............................................................................... 26
B. Tinjauan Pustaka ..................................................................................... 27
C. Metodologi Praktikum............................................................................. 32
1. Waktu dan Tempat Praktikum ............................................................ 32
2. Alat ..................................................................................................... 32
3. Bahan .................................................................................................. 32
4. Cara Kerja ........................................................................................... 33
D. Hasil Pengamatan dan Pembahasan ........................................................ 36
1. Hasil Pengamatan ............................................................................... 36
2. Pembahasan ........................................................................................ 57
E. Kesimpulan dan Saran ............................................................................. 64
1. Kesimpulan ......................................................................................... 64

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 2. Saran ................................................................................................... 64
DAFTAR PUSTAKA

III. ANALISIS ALGAE DAN PROTOZOA DARI ALAM ............................. 65

A. Pendahuluan ............................................................................................ 65
1. Latar Belakang .................................................................................... 65
2. Tujuan Praktikum ............................................................................... 66
B. Tinjauan Pustaka ..................................................................................... 67
C. Metodologi Praktikum............................................................................. 71
1. Waktu dan Tempat Praktikum ............................................................ 71
2. Alat ..................................................................................................... 71
3. Bahan .................................................................................................. 71
4. Cara Kerja ........................................................................................... 71
D. Hasil Pengamatan dan Pembahasan ........................................................ 73
1. Hasil Pengamatan ............................................................................... 73
2. Pembahasan ........................................................................................ 80
E. Kesimpulan dan Saran ............................................................................. 90
1. Kesimpulan ......................................................................................... 90
2. Saran ................................................................................................... 91
DAFTAR PUSTAKA

IV. PENGENALAN MESOFAUNA, MAKROFAUNA DAN MIKORIZA ... 92

A. Pendahuluan ............................................................................................ 92
1. Latar Belakang .................................................................................... 92
2. Tujuan Praktikum ............................................................................... 93
B. Tinjauan Pustaka ..................................................................................... 94
C. Metodologi Praktikum............................................................................. 96
1. Waktu dan Tempat Praktikum ............................................................ 96
2. Alat ..................................................................................................... 96
3. Bahan .................................................................................................. 96
4. Cara Kerja ........................................................................................... 96
D. Hasil Pengamatan dan Pembahasan ........................................................ 97
1. Hasil Pengamatan ............................................................................... 97
2. Pembahasan ........................................................................................ 102
E. Kesimpulan dan Saran ............................................................................. 105
1. Kesimpulan ......................................................................................... 105
2. Saran ................................................................................................... 105
DAFTAR PUSTAKA

V. MIKROBIOLOGI TERAPAN .................................................................... 106

A. Pendahuluan ............................................................................................ 106
1. Latar Belakang .................................................................................... 106
2. Tujuan Praktikum ............................................................................... 107

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 B. Tinjauan Pustaka ..................................................................................... 108
C. Metodologi Praktikum............................................................................. 111
1. Waktu dan Tempat Praktikum ............................................................ 111
2. Alat ..................................................................................................... 111
3. Bahan .................................................................................................. 111
4. Cara Kerja ........................................................................................... 112
D. Hasil Pengamatan dan Pembahasan ........................................................ 114
1. Hasil Pengamatan ............................................................................... 114
2. Pembahasan ........................................................................................ 119
E. Kesimpulan dan Saran ............................................................................. 125
1. Kesimpulan ......................................................................................... 125
2. Saran ................................................................................................... 125
DAFTAR PUSTAKA

LAMPIRAN

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 DAFTAR TABEL

Tabel 1.1 Pengenalan Alat Elektrik .................................................................... 11

Tabel 1.2 Pengenalan Alat Non Elektrik ............................................................ 14
Tabel 2.1 Hasil Pengamatan Koloni dan Identifikasi Mikroba ........................... 36
Tabel 2.2 Hasil Purifikasi pada Media Miring .................................................... 43
Tabel 2.3 Hasil Purifikasi dan Streak 4 Quadran ................................................ 49
Tabel 2.4 Hasil Pewarnaan Gram........................................................................ 56
Tabel 3.1 Identifikasi Alga .................................................................................. 73
Tabel 3.2 Hasil Pengamatan Protozoa ................................................................ 76
Tabel 3.3 Identifikasi Morfologi Azolla sp. ........................................................ 79
Tabel 3.4 Identifikasi Anabaena azollae ............................................................. 79
Tabel 4.1 Hasil Identifikasi Mesofauna dan Makrofauna ................................... 97
Tabel 5.1 Hasil Pengamatan Nata ....................................................................... 114
Tabel 5.2 Hasil Uji Organoleptik Berbagai Macam Jenis Nata .......................... 116
Tabel 5.3 Hasil Pengamatan Mikoriza ................................................................ 118

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