IR - PERPUSTAKAAN UNIVERSITAS AIRLANGGA

ABSTRAK

MEKANISME PROTEKSI NANO KURKUMIN TERHADAP STRES

OKSIDATIF DAN APOPTOSIS SEL GRANULOSA OVARIUM TIKUS
PUTIH (Rattus norvegicus) YANG DIPAPAR
TIMBAL ASETAT (PbAc)

Anis Satus Syarifah

Pendahuluan: Paparan timbal asetat (PbAc) menyebabkan stress oksidatif sel

granulosa ovarium. Nano kurkumin dapat menghambat stres oksidatif yang sangat
efektif, karena memiliki kelarutan, stabilitas, dan bioavailabilitas lebih baik dan
memiliki potensi antioksidan lebih kuat dibanding dengan kurkumin. Tujuan
penelitian ini menjelaskan mekanisme proteksi nano kurkumin terhadap progresivitas
stress oksidatif dan apoptosis sel granulosa ovarium tikus putih (Rattus norvegicus)
yang dipapar PbAc.
Metode: Jenis penelitian ini true experimental dengan rancangan randomized post
test only control group design. Replikasi penelitian ini 45 tikus putih betina dibagi
menjadi 5 kelompok: Kelompok kontrol negative diberikan minyak jagung dan
aquabidest saja, kelompok kontrol positive diberikan minyak jagung dan Pb 40 mg/kg
bb/hari), tiga kelompok perlakuan secara berurutan diberikan nano kurkumin 50, 100
dan 200 mg/kg bb/hari yang dilarutkan dalam minyak jagung dan diberikan Pb 40
mg/kg bb/hari. Intervensi diberikan secara oral selama 26 hari. Hari ke 27 tikus
dikorbankan diambil ovariumnya. Analisis Imunohistokimia dilakukan untuk
mengetahui ekspresi SOD, GPx, MDA, caspase-3 dan apoptosis sel granulosa
ovarium. Data dipresentasikan dengan rerata ± standard deviation. Uji perbedaan
menggunakan Kruskall-Wallis Test untuk mengetahui perbedaan antar kelompok,
dilanjutkan dengan Mann-Whitney test untuk mengetahui perbedaan diantara
kelompok yang ada.

Hasil: Hasil penelitian menunjukkan bahwa rerata ekspresi SOD sel granulosa
ovarium tikus putih (Rattus norvegicus) tertinggi pada P3 (15,00 ± 12,61) dan
terendah pada K+ (0,35 ± 0,43). Rerata ekspresi GPx tertinggi pada P3 (3,51 ± 0,60)
dan hampir sama dengan kelompok K- (3,51 ± 0,54), serta nilai terendah pada K+
(2,34 ± 0,32). Rerata ekspresi MDA tertinggi pada K+ (94,19 ± 40,41) dan terendah
pada K- (1,48 ± 1,38). Rerata ekpresi caspase-3 tertinggi pada K+ (45,08 ± 42,19)
dan terendah pada P3 (0,09 ± 0,14). Rerata apoptosis tertinggi pada K+ (4,31 ± 0,83)
dan terendah pada P3 (1,99 ± 1,24). Penelitian ini membuktikan bahwa nano
kurkumin menyebabkan peningkatan ekspresi SOD dan GPx, peningkatan SOD
mempengaruhi penurunan MDA dan caspase-3 serta penurunan caspase-3
berpengaruh terhadap penurunan apoptosis, sedangkan peningkatan GPx tidak
memberikan pengaruh terhadap penurunan MDA, caspase-3 dan apoptosis sel
granulosa ovarium tikus putih yang dipapar PbAc

Kesimpulan: Nano kurkumin mengandung antioksidan dapat sebagai proteksi stress

oksidatif dan apoptosis sel granulosa ovarium yang dipapar PbAc
Kata kunci: Nano kurkumin, proteksi, timbal asetat, stress oksidatif, apoptosis, sel
granulosa ovarium
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 IR - PERPUSTAKAAN UNIVERSITAS AIRLANGGA

ABSTRACT

NANOCURCUMIN PROTECTIVE MECHANISM AGAINST OXIDATIVE

STRESS AND APOPTOSIS OF WHITE RATS (Rattus norvegicus) OVARIAN
GRANULOSA CELL OF LEAD ACETATE (PbAc)-EXPOSED

Anis Satus Syarifah

Introduction: Exposure to lead acetate (PbAc) causes oxidative stress of ovarian

granulosa cells. Nanocurcumin can inhibit oxidative stress very effectively because it
has better solubility, stability, and bioavailability and has stronger antioxidant
potential than curcumin. The aim of this study was to reveal the protective mechanism
of nanocurcumin against progression of oxidative stress and apoptosis of ovary
granulosa cells in white rats (Rattus norvegicus) exposed to PbAc.

Methods: This study was a true experimental study using randomized posttest only
control group design. This study was conducted on 45 female white rats divided into 5
groups: negative control group receiving only corn oil and distilled water, a positive
control group receiving corn oil and Pb of 40 mg/kg bw/day, and three treatment
groups, receiving respectively 50, 100 and 200 mg/kg bw/day of nanocurcumin
dissolved in corn oil and receiving Pb of 40 mg/kg bw/day. The intervention was
provided orally for 26 days. On day 27 the rats were sacrificed and their ovaries were
removed. Immunohistochemical analysis was performed to determine the expression
of SOD, GPx, MDA, caspase-3 and ovarian granulosa cell apoptosis. Data are
presented as mean ± standard deviation. The comparative test used was Kruskall-
Wallis Test to determine the differences between groups, followed by Mann-Whitney
test to determine differences between the groups.

Results: The highest mean of SOD expression in ovarian granulosa cells of the white
rats (Rattus norvegicus) was at P3 (15.00 ± 12.61) and the lowest was at K+ (0.35 ±
0.43). The highest mean of GPx expression was at P3 (3.51 ± 0.60) and was almost
similar to that in K- group (3.51 ± 0.54), and the lowest was at K+ (2.34 ± 0.32).
The highest mean of MDA expression was at K+ (94.19 ± 40.41) and the lowest
was at K- (1.48 ± 1.38). The highest mean of caspase-3 expression was at K+ (45.08
± 42.19) and the lowest was at P3 (0.09 ± 0.14). The highest mean of apoptosis
was at K+ (4.31 ± 0.83) and the lowest was at P3 (1.99 ± 1.24). This study proved
that nano curcumin causes an increase in SOD and GPx expression, an increase in
SOD affects a decrease in MDA and caspase-3 and a decrease in caspase-3 affects a
decrease in apoptosis, while an increase in GPx does not have an effect on decreasing
MDA, caspase-3 and ovarian granulosa cell apoptosis white rats exposed to PbAc.

Conclusion: Nanocurcumin contains antioxidants that can provide protection against

oxidative stress and apoptosis in ovarian granulosa cells exposed to PbAc

Keywords: Nanocurcumin, protection, lead acetate, oxidative stress, apoptosis,

ovarian granulosa cells

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DISERTASI MEKANISME PROTEKSI NANO ... ANIS SATUS SYARIFAH