Trial HPV Rsud Kanujoso (2023-04-17-20230417 - 122037 - Tes HPV 2-1)

PEMERINTAH PROVINSI KALIMANTAN TIMUR
“RUMAH SAKIT UMUM DAERAH “Dr. KANUJOSO DJATIWIBOWO“

Jl. Letjend. M.T. Haryono Telp. 873901-873539–873837 Fax. (0542) 873836
Email address : rskd@kaltimprov.go.id / kepegawaian.kanujoso14@gmail.com
B A L I K P A P A N - 76126
Telah dilakukan pengujian kit deteksi dini virus HPV menggunakan CerviScan HPV-hr qPCR Biofarma
pada tanggal 17 April 2023 bertempat di Laboratorium Mikrobiologi RSUD Kanujoso Djatiwibowo,
Balikpapan. Sampel yang digunakan berupa urine probandus dengan rincian:
1. 7 sampel pasien dan analis lab
2. 5 sampel dokter
3. 1 kontrol positif yang terdapat dalam box kit
4. 1 kontrol negatif yang terdapat dalam box kit
Adapun hasil yang didapatkan tercantum dalam tabel dan grafik analisis sebagai berikut:
Standard report
Experiment summary
Experiment name 20230417_122037_TES HPV 2
Run start 4/17/2023, 12:21:12 PM +08:00 UTC
Run finish 4/17/2023, 1:29:24 PM +08:00 UTC
User Administrator Administrator
Notes
Run on software version 1.1.0.4
Run on instrument 1018147 (s/n COM3)
qPCR protocol
Rotor type 72-Well Rotor
Reaction volume 20 µl
Phase Description
Hold Hold at 95°C, 00:02:00
Cycling(45 repeats) Step 1: Hold at 95°C, 00:00:01
Step 2: Hold at 60°C, 00:00:10, acquiring on Green, Yellow, Orange, Red,
Crimson
Major errors
Error-code
Analysis specific information
Standard Report from the Basic Plugin

Created by Administrator Administrator (admin) on 4/17/2023, 1:42:10 PM +08:00 UTC
Q-Rex (Version 1.1.0.4)
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Normalized Amplification Plot
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Analysis Settings
Target Green
Filter Data Normalization
Digital Filter Light Dynamic Tube Yes

Remove non-amplified curves with Unchecked Noise slope Correction Unchecked
fluorescence change -- Ignore first Cycles --
reaction efficiency -- Adjust Take-off Points Unchecked
Crop Cycles Unchecked If take-off point: --
Remove data before cycle: -- use take-off cycle: --
Remove data after cycle: --
Cq Calculation Melt Peak Analysis

Threshold 0.0399 Determine Melt Peaks --
Threshold start cycle 1 Peak Threshold --
Tm Threshold --
Target Yellow



Tm Threshold --
Target Orange

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Tm Threshold --
Target Red


Tm Threshold --
Target Crimson


Tm Threshold --
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Tube Results
Tube Sample name Sample groups Sample type
Acquisition Target Cq Take-off Eff. Tm °C
1 DYAH Sample
Crimson Crimson 28.14 26.50 1.76
Green Green -- -- --
Orange Orange -- -- --
Red Red -- -- --
Yellow Yellow -- -- --
2 PIPIT Sample
Red Red -- -- --
3 MERRY Sample
Red Red -- -- --
4 DR RINI Sample
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Red Red -- -- --
5 DR ARI Sample
Red Red -- -- --
6 DR MARTIN Sample
Red Red -- -- --
7 DR RIA Sample
Red Red -- -- --
8 EPY Sample
Red Red -- -- --
9 TYO Sample
Red Red -- -- --
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10 SANAH Sample
Red Red -- -- --
11 DR OTIN Sample
Red Red -- -- --
12 MASRIPAH Sample
Red Red -- -- --
13 NTC NTC
Crimson Crimson -- -- --
Red Red -- -- --
14 PC PC
Green Green 30.01 24.20 1.50
Orange Orange 27.97 24.00 1.73
Red Red 26.83 23.20 1.65
Yellow Yellow 26.03 24.00 1.74
Sample Results
Sample name Sample groups Sample type
Acquisition Target Sample Cq Cq SD
DYAH Sample
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Crimson Crimson 28.14 --
Green Green -- --
Orange Orange -- --
Red Red -- --
Yellow Yellow -- --
PIPIT Sample
Green Green -- --
Orange Orange -- --
Red Red -- --
Yellow Yellow -- --
MERRY Sample
Green Green -- --
Orange Orange -- --
Red Red -- --
Yellow Yellow -- --
DR RINI Sample
Green Green -- --
Orange Orange -- --
Red Red -- --
Yellow Yellow -- --
DR ARI Sample
Green Green -- --
Orange Orange -- --
Red Red -- --
Yellow Yellow -- --
DR MARTIN Sample
Green Green -- --
Orange Orange -- --
Red Red -- --
Yellow Yellow -- --
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DR RIA Sample
Green Green -- --
Orange Orange -- --
Red Red -- --
Yellow Yellow -- --
EPY Sample
Green Green -- --
Orange Orange -- --
Red Red -- --
Yellow Yellow -- --
TYO Sample
Green Green -- --
Orange Orange -- --
Red Red -- --
Yellow Yellow -- --
SANAH Sample
Green Green -- --
Orange Orange -- --
Red Red -- --
Yellow Yellow -- --
DR OTIN Sample
Green Green -- --
Orange Orange -- --
Red Red -- --
Yellow Yellow -- --
MASRIPAH Sample
Green Green -- --
Orange Orange -- --
Red Red -- --
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Yellow Yellow -- --
NTC NTC
Crimson Crimson -- --
Green Green -- --
Orange Orange -- --
Red Red -- --
Yellow Yellow -- --
PC PC
Green Green 30.01 --
Orange Orange 27.97 --
Red Red 26.83 --
Yellow Yellow 26.03 --
Berdasarkan tabel dan grafik tersebut pada Kontrol Positif (PC) terbaca positif dan Kontrol Negatif (NTC)
terbaca negatif disemua channel pembacaan. Selain itu Internal kontrol (Cy5.5/Crimson) juga terbaca positif di semua
sampel. Grafik pada semua sampel probandus dari berbagai gen target yang telah ditentukan menunjukkan kurva yang
berbentuk landai atau tidak sigmoid, sehingga dinyatakan negatif..
KESIMPULAN
Pengujian kit deteksi dini virus HPV dengan menggunakan Kit CerviScan HPV-hr qPCR Biofarma
menghasilkan data yang baik dan kit tersebut dapat digunakan.
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Trial HPV Rsud Kanujoso (2023-04-17-20230417 - 122037 - Tes HPV 2-1)

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Trial HPV Rsud Kanujoso (2023-04-17-20230417 - 122037 - Tes HPV 2-1)

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Hak Cipta:

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PEMERINTAH PROVINSI KALIMANTAN TIMUR

“RUMAH SAKIT UMUM DAERAH “Dr. KANUJOSO DJATIWIBOWO“

Analysis specific information

Standard Report from the Basic Plugin

Q-Rex (Version 1.1.0.4)

Created by Administrator Administrator (admin) on 4/17/2023, 1:42:10 PM +08:00 UTC

Q-Rex (Version 1.1.0.4)

Created by Administrator Administrator (admin) on 4/17/2023, 1:42:10 PM +08:00 UTC

Q-Rex (Version 1.1.0.4)

Digital Filter Light Dynamic Tube Yes

Remove data before cycle: -- use take-off cycle: --

Remove data after cycle: --

Cq Calculation Melt Peak Analysis

Filter Data Normalization

Remove data before cycle: -- use take-off cycle: --

Cq Calculation Melt Peak Analysis

Filter Data Normalization

Digital Filter Light Dynamic Tube Yes

Created by Administrator Administrator (admin) on 4/17/2023, 1:42:10 PM +08:00 UTC

Q-Rex (Version 1.1.0.4)

Cq Calculation Melt Peak Analysis

Filter Data Normalization

Remove data before cycle: -- use take-off cycle: --

Remove data after cycle: --

Cq Calculation Melt Peak Analysis

Filter Data Normalization

Digital Filter Light Dynamic Tube Yes

Remove data before cycle: -- use take-off cycle: --

Remove data after cycle: --

Cq Calculation Melt Peak Analysis

Created by Administrator Administrator (admin) on 4/17/2023, 1:42:10 PM +08:00 UTC

Q-Rex (Version 1.1.0.4)

Acquisition Target Cq Take-off Eff. Tm °C

Created by Administrator Administrator (admin) on 4/17/2023, 1:42:10 PM +08:00 UTC

Q-Rex (Version 1.1.0.4)

Q-Rex (Version 1.1.0.4)

Acquisition Target Sample Cq Cq SD

Created by Administrator Administrator (admin) on 4/17/2023, 1:42:10 PM +08:00 UTC

Q-Rex (Version 1.1.0.4)

Created by Administrator Administrator (admin) on 4/17/2023, 1:42:10 PM +08:00 UTC

Q-Rex (Version 1.1.0.4)

Created by Administrator Administrator (admin) on 4/17/2023, 1:42:10 PM +08:00 UTC

Q-Rex (Version 1.1.0.4)

Created by Administrator Administrator (admin) on 4/17/2023, 1:42:10 PM +08:00 UTC

Q-Rex (Version 1.1.0.4)

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