KELOMPOK 3

01 Rika Febriyatul Nur Anisa 202210410311058

02 Zakiyatul Fakhirah 202210410311059

03 Muhammad Hisyam Dzakwani 202210410311061

04 Ailsa Nasywa Nabila 202210410311063

05 Regita Nanda Puspita 202210410311066

Morfologi Bakteri &
Morfologi Fungi
 Tema VI

Morfologi
Bakteri
Pewarnaan Bakteri Gram,
Endospora, dan Tahan Asam
 TUJUAN PRAKTIKUM

1. Mahasiswa mampu menginterprestasikan morfologi bakteri

2. Mahasiswa mampu melakukan identifikasi bakteri melalui pewarnaan sederhana dan
gram sesuai dengan prosedur praktikum
3. Mahasiswa mampu menjelaskan perbedaan bakteri gram positif dan negatif
4. Mahasiswa mampu memahami dan melakukan pewarnaan endospora
5. Mahasiswa mampu mengidentifikasi bakteri yang termasuk bakteri endospora
6. Mahasiswa mampu mengidentifikasi perbedaan bakteri tahan asam dan non tahan
asam
 Prosedur
1.Pembuatan Sediaan (Smear)

01

02 Teteskan pewarna kristal violet

03 Teteskan cairan lugol

04 Bilas dengan alcohol 96%

05 Teteskan pewarna safranin

06 Periksa dibawah mikroskop,tambahkan minyak immerse 1 tetes

untuk perbesaran 100x
 Prosedur Pembuatan Sediaan (Smear)

01 Bersihkan gelas objek dengan kapas

Lewatkan di atas api atau alcohol untuk
02 menghilangkan lemak, lalu dinginkan
Siapkan ose, setiap menggunakan ose dipanaskan terlebih
03 dahulu di api bunsen dan dinginkan

Buat sediaan tidak terlalu tebal dan tidak terlalu tipis dengan cara:
04 1. Biakan padat: panaskan ose ambil aquadest steril dan teteskan satuose pada gelas
obyek, kemudian ambil bakteri padat sedikit denganose yang telah dipanaskan dan
suspensikan dengan aquadest digelas obyek dan ratakan.
2. Biakan cair panaskan ose ambil satu ose bakteri dan teteskan pada gelas obyek dan
ratakan.
05 Biarkan sediaan kering di udara, setelah kering lakukan fiksasi dengan cara lewatkan
sediaan di atas api sebanyak 3x dan sediaan siap diwarnai.
Alat dan Bahan Pembuatan Sediaan (smear)

Mikrokop Cover Glass Timer

Sediaan Bakteri
Kawat ose kertas hisab pembentuk spora pada
gelas obyek
 Minyak imersi Aquadest
Methhyylen blue
Alat dan Bahan Pembuatan sediaan (smear)

bunsen Timer
sediaan bakteri

kertas hisab
 Prosedur Pewarnaan sederhana

01 Siapkan sediaan bakteri pada gelas obyek

02 Tuangi sediaan dengan pewarna Methylene blue atau Safranin

secukupnya,diamkan selama ½-1 menit

03 Buang sisa pewarna dan bilas dengan air, kemudian

keringkanmenggunakan kertas penghisap.
Lihat di bawah mikroskop dengan lensa obyektif perbesaran 100x (beri
04 1tetes minyak immersi).
 2.Pewarnaan Sederhana

01 Siapkan sediaan bakteri pada gelas obyek

02 Teteskan pewarna kristal violet

03 Teteskan cairan lugol

04 Bilas dengan alcohol 96%

05 Teteskan pewarna safranin

06 Periksa dibawah mikroskop,tambahkan minyak immerse 1 tetes

untuk perbesaran 100x
 Alat dan Bahan Pewarnaan Gram

Mikrokop Cover Glass Wadah Buangan

Biakan bakteri pada

Kertas Hisap
gelas obyek
Pewarna Crystal-violet Safranin Lugol

Alkohol 96% Aquadest Minyak imersi

 3.Prosedur Pewarnaan Gram

01 Siapkan sediaan bakteri pada gelas obyek

02 Teteskan pewarna kristal violet

03 Teteskan cairan lugol

04 Bilas dengan alcohol 96%

05 Teteskan pewarna safranin

06 Periksa dibawah mikroskop,tambahkan minyak immerse 1 tetes

untuk perbesaran 100x
 Alat dan Bahan Pewarnaan Endospora

Mikrokop Cover Glass Timer

Sediaan Bakteri
Kertas Hisap pembentuk spora pada
gelas obyek
Mallachite green Safranin Aquadest
 4. Prosedur Pewarnaan Endospora

01 Siapkan sediaan bakteri pada gelas obyek

02 Tetetskan sediaan dengan malachite green

03 Panaskan diatas Bunsen selama 5 menit

04 Buang sisa pewarna dan bilas dengan air

Teteskan sediaan safranin dan bilsa dengan

05
air,keringkan sediaan

06 Periksa dibawah mikroskop,tambahkan minyak immerse 1 tetes

untuk perbesaran 100x
 5.Prosedur Pewarnaan Tahan Asam

01 Buat sediaan dari bahan pemeriksaan sputum pada

gelas obyek

02 Tuangi sediaan dengan carbol-fuchsin dan panaskan

selama 5 menit,dijaga jangan sampai mendidih.

03 Tuangi sediaan dengan alkohol asam sampai warna

cat luntur.
Tuangi sediaan dengan methylene blue,diamkan ½
04 menit
Teteskan sediaan safranin dan bilsa dengan
05
air,keringkan sediaan

06 Keringkan sediaan dengan kertas penghisap/tissue dan lihat pada

mikroskop .
Morfologi Fungi
Pewarnaan LPCB
 Tujuan Praktikum

1. Mahasiswa mampu menjelaskan morfologi fungi

2. Mahasiswa mampu melakukan pemeriksaan jamur dengan pewarnaan LPCB/KOH

3. Mahasiswa mampu menginterpretasikan hasil pemeriksaan fungi berdasarkan

ciri – ciri mikroskopik

4. Mahasiswa mampu menjelaskan perbedaan jenis dan bagian – bagian fungi

INTERPRETASI

Pewarnaan gram

gram positif = berwarna ungu

berbentuk = coccus
perbesaran = 100x lensa obyektif
pewarnaa gram menggunakan kristal violet
(ungu) dan safranin (merah)
tujuan pewarnaan gram : untuk
mengidentifikasi bakteri
 Pewarnaan endospore
Letak spora :
sentral

Sel
vegetatif

(perbesaran 100x lensa obyektif)

jenis pewarna yang dapat menembus dan mewarnai endospore

adalah malachite green atau carbol fuchsin.

Tujuan pewarnaan endospore : untuk mengidentifikasi bakteri yang

mampu menghasilkan spora.
 Fungi

(perbesaran 100x lensa obyektif)

berwarna biru
KESIMPULAN
Organelles Found in the Cytoplasm

Ribosomes Lysosomes
It’s the biggest planet Despite being red,
of them all Mars is a cold place

Vacuoles Peroxisomes
It’s a gas giant and Venus is the second
has several rings planet from the Sun
 Cell Functions

Mars Mercury
Despite being red, is It’s the smallest
a cold place planet of them all

Venus Jupiter
Venus has a beautiful It’s a gas giant and
name has several rings
02
Animal and Plant
Cells
Enter a subtitle here if you need it
 Cellular Study Process

01 02 03

Theories Practice Studies

Mercury is the smallest Venus is the second Despite being red, Mars
planet of them all planet from the Sun is a cold place

04 05 06
Materials Observations Results
Earth is the third planet Saturn is a gas giant and Jupiter is the biggest
from the Sun has several rings planet of them all
 Cellular Process

Animal Cell
Despite being red, it’s
actually a cold place

Plant Cell Mutation

Venus is the second It’s the closest planet to
planet from the Sun the Sun
 Differences between:

Animals Cells Plants Cells

● Here you can state one of
your qualities
● Here you can state one of
your qualities
● Here you can state one of
your qualities
● Here you can state one of
your qualities
● Here you can state one of
your qualities
● Here you can state one of
your qualities
● Here you can state one of
your qualities
● Here you can state one of
your qualities
 “This is a quote, words full of wisdom
that someone important said and can make
the reader get inspired.”
—SOMEONE FAMOUS
A picture is worth a
thousand words
 Particular Characteristics of Cells

Plants Cells
Venus has a beautiful name
and is the second planet
from the Sun

Animals Cells
It’s the closest planet to the
Sun and the smallest one in
the Solar System
 Parts of the Animal Cell

Nucleus Mitochondria
Despite being red, it’s It’s the closest planet to
actually a cold place the Sun

Cytoplasm Membrane
Venus is the second Earth is the planet
planet from the Sun where we live on
 Parts of the Plant Cell

Nucleus Cell Wall

Despite being red, is It’s the closest planet to
actually a cold place the Sun

Mitochondria Membrane
Venus is the second Jupiter is the biggest
planet from the Sun planet of them all

Cytoplasm Vacuole Chloroplast

Neptune is very far Earth is the planet Saturn is a gas giant
away from Earth where we live on and has rings
 Functions of the Cell Parts
Cell Structure Function (Job) Cell Structure Function (Job)

Mercury is the smallest It’s the biggest planet

Nucleus Cell Wall
planet in the Solar System

Venus is the second It’s now considered a

Cytoplasm Chloroplast
planet from the Sun dwarf planet

Despite being red, It’s located in the main

Cell Surface membrane Vacuole
Mars is cold asteroid belt

Saturn is a gas giant

Mitochondria
and has rings
 Cytoplasmic Organelles

55%
35% 75%

Golgi Liposomes Mitochondria

Venus is the second planet It’s the closest planet to Despite being red, Mars is
from the Sun the Sun and the smallest a cold place
150,000
Big numbers catch your audience’s attention
 Tema VII

Morfologi
Fungi
Pewarnaan LPCB
 Teori Dasar

Jamur (fungi) adalah sel eukariotik yang

tidak memiliki klorofil, tumbuh sebagai
hifa, memiliki dinding sel yang
mengandung kitin dan bersifat heterotrof.
Jamur memiliki morfologi dan struktur
yang dapat dilihat dengan cara pewarnaan
menggunakan larutan Lacto Phenol
Cotton Blue (LPCB) atau pemeriksaan
langsung menggunakan preparat KOH.
 Alat dan Bahan

Pembenihan C.albicans Pembenihan Jamur Lacto Phenol Cotton

pada SDA Filamentous pada SDA Blue (LPCB)

Kuku Dan Potongan

KOH/NaOH 10 %
Rambut
 Prosedur Kerja

2
Pemeriksaan dengan
pewarnaan menggunakan
LPCB

1
Pemeriksaan Langsung
Secara Miroskopis
 Pemeriksaan Langsung Secara Mikroskopis

1 3 5
Letakkan bahan
pemeriksa pada gelas Panaskan diatas api spiritus Perhatikan kemungkinan
obyek adanya sel yeast, budding
2 4
cells, mycelium atau hyphae
Teteskan 1 tetes
Setelah dingin, periksalah di
KOH/NaOH 10 % pada
bawah mikroskop dengan
bahan pemeriksa dan
perbesaran lensa obyektif
tutuplah dengan gelas
10-40 x dan intensitas sinar
penutup
rendah
 Pemeriksaan Dengan Pewarnaan Menggunakan
LPCB

1 3
Ambillah biakan C. albicans atau periksalah di bawah
kultur jamur filamentous mikroskop dengan
menggunakan ose dan letakkan perbesaran lensa obyektif
pada gelas obyek 2 40 x 4
Teteskan LPCB pada Perhatikan morfologinya (
gelas obyek dan tutup hifa bersepta atau tidak,
dengan gelas penutup spora, yeast, pseudohypha
)
 Main Cells Studies

Mars Venus Jupiter

Despite being red, it’s Venus is the second It’s the biggest planet
a cold place planet from the Sun of them all

Mercury Saturn Neptune

It’s the closest planet Saturn is a gas giant It’s the farthest planet
to the Sun and has rings from the Sun
 Materials Timetable

Objets 8:00 9:00 10:00 11:00 12:00

Not used Used

 The Most Important Laboratories

Saturn Neptune
It’s a gas giant and It’s the farthest planet
has several rings from the Sun

Venus Mars
Venus is the second Mars is actually a
planet from the Sun very cold place
 Percentages

20% Mars
Mars is actually a
very cold place

80% Venus
Venus is the second
planet from the Sun

Follow the link in the graph to modify its data and then
paste the new one here. For more info, click here
150,000
Big numbers catch your audience’s attention
 Studies Follow-ups
Study 1 Study 2 Study 3

Mercury Mars Neptune

It’s the closest planet Despite being red, It’s the farthest planet
to the Sun Mars is a cold place from the Sun

Venus Jupiter Saturn

It’s the second planet Jupiter is the biggest It’s composed of
from the Sun planet hydrogen & helium
 Studies Steps

Mercury Venus
Mercury is the closest Venus has a beautiful
planet to the Sun name, but it’s hot

Mars Neptune
Despite being red, Mars is It’s the farthest planet
actually cold from the Sun
 Our Team

Jenna Doe Timmy Jimmy Susan Bones

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