Teknik Sterilisasi dan Asepsis

Mutia Oktori Yelfitri & Brilianti Vica

Dewi A.S
Sterilisasi
 Pengertian

Proses yang secara efektif membunuh atau

menghilangkan mikroorganisme yang dapat
berpindah (jamur, bakteri, dan virus) dari
permukaan peralatan (Rahmatullah dkk, 2007).
 Kategori Peralatan Medis

01 Kritis 02 Semikritis 03 Non Kritis

 Metode Sterilisasi
Sterilisasi Uap
dibawah Unsatured
tekanan Chemical
(Autoclaving) Vapor

Dry Heat
Sterilization
Autoclaving Prinsip kerja :
a. Pemanasan air di ruang
tertutup
b. Titik didih air naik
Golongan c. Autoclave akan
barang memanfaatkan tekanan
cooker dengan
critical dan
ruangan double-walled
semi critical
d. Uap bersirkulasi
dibawah tekanan dalam
chamber (tempat
objek yang disterilkan)
e. Setelah sterilisasi
selesai, pengeringan
muatan bersamaan
dengan evakuasi uap
 Dry Heat Sterilization
Digunakan untuk material
yang rusak oleh sterilisasi
Terdiri dari 2 tipe :
panas dan lembab
+ : Biaya operasional rendah
dan tidak korosif Static air
- : proses lama dan (sterilisasi oven)
temperatur tinggi

Parameter Sterilisasi Forced-air (rapid

Dry Heat : heat-transfer)
Unsaturated Chemical Vapor
Melibatkan pemanasan larutan kimia
alkohol primer dengan 0,23% formaldehyde
pada ruangan bertekanan tertutup

Menghasilkan korosi lebih sedikit

dibandingkan sterilisasi uap

Ketika melakukan sterilisasi, instrumen

harus dalam keadaan kering

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 Langkah Sterilisasi
1 2 3 4

Letakkan
Bersihkan Keringkan Letakkan instrumen yang
instrumen Instrument instrument telah bersih di
dalam wadah kantong
tertutup sterilisasi sesuai
8 7 6 ADA 5

Letakkan instrumen Instrumen yang telah Atur suhu Masukkan ke

yang telah steril pada steril disimpan pada sesuai yang dalam
rak penyimpanan tempat yang tertutup dibutuhkan stelilizator
steril (autoclave)
Asepsis
 Pengertian
Kondisi dimana tidak dijumpai organisme
patogen dan merupakan prosedur klinis
yang dilakukan untuk mencegah
kontaminasi dari luka dan bagian tubuh
lainnya
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