PEMERIKSAAN PREALBUMIN METODE IMUNOTURBIDIMETRI

Presentan: Kania Retnaning Astuti Pembimbing: M.I Diah Pramudianti,dr, MSc, SpPK

PPDS PATOLOGI KLINIK FK UNS / RSDM SURAKARTA 2013

TAHAP DASAR: ORIENTASI

NO 1 SUB STASE KIMIA 1 TUGAS METODE 1 WAKTU 1 AGUSTUS 2013 TOPIK PEMERIKSAAN PREALBUMIN METODE IMUNOTURBIDIMETRI

PENDAHULUAN

PENDAHULUAN PREALBUMIN

PENDAHULUAN KADAR PREALBUMIN

PENDAHULUAN
RADIAL IMMUNOASSAY

ELISA IMUNONEFELOMETRI

METODE PEMERIKSAAN PREALBUMIN

PRA ANALITIK

ANALITIK

PASKA ANALITIK
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PRA ANALITIK

TUJUAN :

MENENTUKAN KADAR PREALBUMIN DALAM SERUM SECARA KUANTITATIF

ALAT DAN BAHAN

SERUM DISIMPAN PADA SUHU 4C DAN -20C
ALAT
Anonim, 2008

BAHAN

WADAH SAMPEL LARUTAN SISTEM KALIBRATOR DAN KONTROL REAGENT CONTAINER ADAPTERS

KIT

REAGEN

R 1: PEG R 2: ANTIBODI ANTI PREALBUMIN

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PERSIAPAN ALAT

KALIBRASI

QUALITY CONTROL (QC)

QC HARIAN, MINIMAL 2 LEVEL KEADAAN TERTENTU YANG MEMBUTUHKAN QC

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ANALITIK PRINSIP KERJA

TURBIDITAS PANJANG GELOMBANG 340 NM ABSORBEN KONSENTRASI PREALBUMIN

KOMPLEKS ANTIGEN-ANTIBODI

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ANALITIK

PRINSIP KERJA IMUNOTURBIDIMETRI (Rock, 1995)

CARA KERJA ADVIA 1800

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PASKA ANALITIK
RENTANG NILAI NILAI NORMAL : 10-40 mg/dL RENTANG NILAI ANALITIK ALAT: 5- (70-80) mg/dL BILIRUBIN HEMOLISIS LIPEMIA

PRESISI

2x PER RUN 2 RUN/ HARI 10 HARI

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SIMPULAN

Prealbumin indikator monitoring efek jangka pendek terapi nutrisi dan kecukupan rencana pemberian nutrisi.

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DAFTAR PUSTAKA
Anonim. 2008. Prealbumin. USA: Siemens Advia Chemistry, pp: 1-10. Collins N. 2001. Advanced in skin and wound care. Proquest Medical Library. 14:235. Gorman L.S., Boosalis M.G. 2010. Nutritional Assessment. In: Bishop M.L., Fody E.P., Schoeff L.E. (eds). Clinical Chemistry: Techniques, Principles, Correlations. 6th edition. Philadelphia: Lippincott William&Wilkins, pp: 654. Johnson A.M., Merlini G., Sheldon J., Ichihara K. 2007. Clinical Indications for Plasma Protein Assay: Transthyretin (prealbumin) in Inflammation and Malnutrition. http://www.ncbi.nlm.nih.gov/pubmed/17378745. (diunduh tanggal 21 Agustus 2013). Monaco H.L., Rizzi M., Coda A. 1995. Structure of a Complex of Two Plasma Proteins: Transthyretin and Retinol Binding Protein. http://www.ncbi.nlm.nih.gov/ pubmed/7754382. (diunduh tanggal 21 Agustus 2013). Orton S. 2010. Immunoassays. In: Bishop M.L., Fody E.P., Schoeff L.E. (eds). Clinical Chemistry: Techniques, Principles, Correlations. 6th edition. Philadelphia: Lippincott William&Wilkins, pp: 189. Rock F. 2005. Absorption, Scatter and Luminescence Techniques in Routine Clinical Biochemistry. In: Crocker J., Burnett D. (eds). The Science of Laboratory Diagnosis. 2nd edition. West Sussex: Wiley, pp: 395. Tymchak L. 2010. Amino Acids and Proteins. In: Bishop M.L., Fody E.P., Schoeff L.E. (eds). Clinical Chemistry: Techniques, Principles, Correlations. 6th edition. Philadelphia: Lippincott William&Wilkins, pp: 238. Unal D., Orhan O., Eroglu C., Kaplan B. 2013. Prealbumin is a more sensitive marker than albumin to assess the nutritional status in patients undergoing 15 radiotherapy for head and neck surgery. Wspolczesna Onkol. 17: 276-280.