Assalamualaikum Wr.

Wb
Oleh Kelompok 5 XII MIPA 8

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Anggota Kelompok
ANDREW AVRILLOSTYA
01 NO ABSEN 03

MUTIARA EMBUN KHARISMA

02 NO ABSEN 20

PUTRI AYU ISNAINI

03 NO ABSEN 26

VIVIOLITA NUR FEBIANI

04 NO ABSEN 34

YULIAN LEGA CAESARIO

05 NO ABSEN 35
BIOTEKNOLOGI
KLONING
 Apa itu Bioteknologi?
Suatu teknik modern untuk mengubah
bahan mentah melalui transformasi biologi
sehingga menjadi produk yang berguna.

Jadi Bioteknologi Adalah :

Pemanfaatan prinsip-prinsip dan rekayasa terhadap
organisme, sistem atau proses biologis untuk manghasilkan
atau meningkatkan potensi organisme maupun menghasilkan
produk dan jasa bagi kepentingan hidup manusia.
 Jenis Bioteknologi
Bioteknologi modern telah
menggunakan teknik rekayasa
tingkat tinggi dan terarah
Bioteknologi
sehingga hasilnya dapat Konvensional
dikendalikan dengan baik.
Teknik yang sering digunakan
adalah dengan melakukan
 Bioteknologi sederhana
manipulasi genetik pada suatu
yang menerapkan ilmu
jasad hidup secara terarah
biologi, biokimia.
sehingga diperoleh hasil sesuai
Bioteknologi
Rekayasa yang terjadi
dengan yang diinginkan.
Modern masih dalam tingkat yang
terbatas. 
TEKNIK KLONING
  Sedangkan kloning
Tujuan utama
Your Picture Here dengan transfer inti
kloning adalah
yaitu pemindahan
untuk mengisolasi
inti sel yang satu ke
gen yang diinginkan
sel lain sehingga
dari seluruh gen
diperoleh individu
yang ada
baru yang memiliki
(kromoson) pada
sifat baru sesuai
organisme donor.
inti yang
Untuk mencapai
diterimanya.
tujuan tersebut,
Kloning dengan
kloning dapat
transfer inti
dilakukan dengan
dilakukan dengan
kloning embrio dan
menggunakan sel
transfer inti.
somatis sebagai
Apa itu Teknik sumber gen. 

Kloning?
Kloning berasal dari bahasa inggris
clonning yang berarti suatu usaha
untuk menciptakan duplikat suatu
organisme melalui proses aseksual.
Ada beberapa dasar yang perlu di kuasai
untuk melakukan Kloning secara
sederhana di antaranya adalah sebagai
berikut:
Preperasi sampel DNA murni 01 04 Penggolongan molekul DNA

Pemotongan DNA murni 02 05 Menyisipkan molekul DNA ke

dalam sel tuan rumah

Analisis ukuran fragmen DNA 03 06 Mengidentifikasi sel yang memiliki

kandungan berupa molekul DNA
rekombinasi
 Proses Kloning

Menyiapkan Menyiapkan Sel telur di Embrio

sel stem sel telur. rangsang agar berkembang
Sel stem Inti sel stem terjadi di dalam rahim
diambil Inti pembelahan Blastosis akan yang bakal
diimplantasika melakukan
selnya yang n ke sel telur. serta menjadi bayi
memiliki melakukan pemisahan diri dengan kode
kandungan pertumbuhan. dan siap genetic hampir
berupa Setelah diimplantasika sama dengan
informasi membelah n ke rahim. sel stem
genetic lalu di menjadi donor.
pisahkan dari embrio
selnya.
K
E
I
T
H

C
A
M
P
B
L
E
 Jenis-Jenis Kloning

Seperti yang telah di jelaskan pada uraian

mengenai cloning di atas, terdapat beberapa jenis
cloning yang biasa kita kenal diantaranya adalah
sebagai berikut.
Kloning DNA rekombinan
berupa pemindahan sebagian rantai DNA yang di
butuhkan dari suatu organisme di salah satu
element replikasi genetic,

Kloning Reproduktif
teknologi yang di gunakan untuk menghasilkan hewan
yang sama,

Kloning Terapeutik
suatu cloning digunakan untuk menghasilkan/
memproduksi embrio manusia bertujuan sebagai
bahan penelitian.
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BAYI TABUNG
Bayi tabung adalah salah satu
metode dalam bioteknologi
modern yang berguna untuk
menyelesaikan masalah
infertilitas. Prosedur bayi
tabung ini dilakukan dengan
cara mengambil sel telur dan
sperma dari dalam tubuh
kemudian dilakukan
pembuahan di media khusus
diluar tubuh.
 Teknik ini dilakukan pada
sebuah medium khusus
yang dilakukan di
laboratorium. IVF
digunakan untuk
menangani masalah
infertilitas seperti kualitas
sperma yang rendah atau
kerusakan tuba fallopi. 

Apa itu Bayi Tabung?

Bayi tabung adalah suatu proses pembuahan sel telur
oleh sel sperma di luar tubuh wanita, tepatnya di
dalam sebuah tabung pembuahan. Setelah sel telur
berhasil dibuahi dan ada dalam fase siap, maka akan
dipindahkan ke dalam rahim. Secara medis proses bayi
tabung disebut dengan in vitro fertilization (IVF).
 TAHAPAN BAYI TABUNG

PEMERIKSAAN STIMULASI OVUM Pematangan Oosit

AWAL
untuk meningkatkan akan diberi suntikan
Memeriksa kadar jumlah sel telur yang human chorionic
hormon estrogen pada diproduksi oleh gonadotropin (hCG)
calon ibu, untuk ovarium. guna pematangan
memastikan bahwa telurnya maksimal.
ovarium daam
keadaan tidur
 TAHAPAN BAYI TABUNG

PENGAMBILAN PEMBUAHAN TELUR PEMINDAHAN

TELUR TELUR
Inseminasi adalah
Pengambilan telur Pemindahan telur
saat dimana sperma
dilakukan yang sudah dibuahi
diperkenalkan ke
menggunakan (embrio) biasanya
telur, kemudian hasil
jarum yang akan dilakukan pada hari
gabungan keduanya
menghisap folikel kelima setelah
dimasukkan ke
dalam ovarium pembuahan, dimana
dalam ruangan
embrio sudah  berada
khusus.
pada fase blastosit..
 Penerapan
Bioteknologi
 BIDANG PANGAN

KONVENSIONAL MODERN

PENGOLAHAN PENGOLAHAN TANAMAN

PRODUK SUSU PRODUK NON-SUSU TRANSGENIK
YOUGHURT KECAP
KEJU TEMPE
MENTEGA TAPE
 Aplikasi Bioteknologi Dalam
Bidang Pangan

Yoghurt merupakan minuman hasil

fermentasi susu menggunakan
bakteri Lactobacillus substilis atau
Lactobacillus bulgaricus.Bakteri yang
YOGURT di manfaatkan mampu mendegradasi
protein dalam susu menjadi asam
laktat. Proses degradasi ini disebut
fermentasi asam laktat dan hasil
akhirnya dinamakan yoghurt.
Aplikasi Bioteknologi Dalam
Bidang Pangan

Keju dibuat dengan bantuan bakteri pada

susu. Bakteri tersebut dikenal sebagai bakteri
asam laktat atau Lactobacillus. Bakteri
Lactobacillus mengubah laktosa menjadi asam
laktat dan menyebabkan susu menggumpal.
Kondisi pH yang rendah membuat susu
mengental. Akibatnya protein pada susu
KEJU berubah menjadi semi solid yang disebut
curd.Proses ini dibantu dengan
menambahkan enzim renin. Enzim renin dapat
diekstrak dari perut anak sapi. Namun, saat ini
enzim renin dapat diproduksi dalam skala
besar dengan menggunakan teknik rekayasa
genetika.
 Aplikasi Bioteknologi Dalam
Bidang Pangan

Tempe terbuat dari kacang kedelai. Karena

terbuat dari kacang kedelai yang merupakan
sumber protein tinggi, tempe juga merupakan
makanan yang mempunyai nilai gizi tinggi. Tempe
dibuat dari kacang kedelai dengan dibantu oleh
aktivitas  jamur Rhizopus oryzae. Kacang kedelai
dicuci  bersih, lalu direbus hingga setengah
TEMPE matang. Kemudian, kacang kedelai setengah
matang direndam dalam air selama kurang lebih
12 jam (semalaman). Dengan direndamnya
kacang kedelai, dapat menciptakan kondisi asam
sehingga mikroba yang biasanya membusukkan
makanan dapat dicegah. Setelah direndam,
kacang kedelai kembali dicuci bersih dan direbus
kembali hingga matang.
 Aplikasi Bioteknologi Dalam
Bidang Pangan

TANAMAN
TRANSGE
NIK
Aplikasi Bioteknologi Dalam Bidang Pertanian
dan Peternakan

Bioteknologi ini bioteknologi hijau,

dilakukan dengan memodifikasi genetik
dan rekayasa genetika untuk memperoleh
varietas unggul, produksi tinggi,
kandungan gizi tinggi, tahan hama,
patogen, dan herbisida. Hal ini
memberikan sumbangan besar terhadap
kemajuan ilmu pemuliaan tanaman (plant
breeding) dan kehidupan manusia bahkan
berdampak pada kemajuan ekonomi
manusia itu sendiri.
 BIOTEKNOLOGI PERTANIAN

A. HIDROPONIK : Metode bercocok

tanam dengan media air. Metodenya
ada 3 : Kultur pasir, air, dan pori (kerikil,
pecahan batu)

B. AEROPONIK : Metode bercocok

tanam dengan cara menyemburkan
kabut air dan nutrisi hara hingga ke akar
tanaman.
 Aplikasi Bioteknologi Dalam
Bidang Pertanian

BUNGA
ANTILAYU
 Aplikasi Bioteknologi Dalam
Bidang Pertanian

PADI
TRANSGENI
K
 Aplikasi Bioteknologi Dalam
Bidang Pertanian

Tomat Flavr Savr merupakan tomat hasil rekayasa

genetika yang memiliki shelf-life lama dapat diciptakan
BUAH dengan menyisipkan gen antibeku dari ikan air dingin
TAHAN ke dalam gen tomat. Gen antibeku ini diperoleh dari
ikan Flounder, yaitu jenis ikan di Antartika yang dapat
BUSUK bertahan hidup dalam kondisi yang sangat dingin. Ikan
Flounder mempunyai gen antibeku yang disebut
dengan gen antisenescens yang dapat menghambat
enzim polygalacturonase (enzim yang mempercepat
kerusakan dinding sel tomat).Polygalacturonase adalah
satu dari beberapa enzim kunci (penting) dalam proses
pelunakan selama pemasakan buah.
Tomat Flavr Savr merupakan tomat hasil rekayasa genetika yang
memiliki shelf-life lama dapat diciptakan dengan menyisipkan gen
antibeku dari ikan air dingin ke dalam gen tomat. Gen antibeku ini
diperoleh dari ikan Flounder, yaitu jenis ikan di Antartika yang dapat
bertahan hidup dalam kondisi yang sangat dingin. Ikan Flounder
mempunyai gen antibeku yang disebut dengan gen antisenescens yang
dapat menghambat enzim polygalacturonase (enzim yang
mempercepat kerusakan dinding sel tomat).Polygalacturonase adalah
satu dari beberapa enzim kunci (penting) dalam proses pelunakan
Dalam buah tomat PRG dan dapat memperpanjang
selama pemasakan buah 
waktu penyimpanan tanpa terjadi pelunakan buah.
Salah satu perusahaan swasta bioteknologi Calgene
telah merakit tomat PRG dengan sifat PK.
Gen antisense polygalacturonase (PG) dengan
promoter CaMV 35S dan gen penanda ketahanan
antibiotik neo (yang mengkode enzim neomycin
phosphotransferase II atau NPTII), ditransformasikan
melalui mediasi vektor A. tumefaciens ke genom
tanaman tomat. Gen PG hasil isolasi
dari elementransposon Tn5 bakteri E.coli. Tomat PRG
 Aplikasi Bioteknologi Dalam
Bidang Pertanian

TANAMAN
KAPAS
ANTISERANG
GA
Aplikasi Bioteknologi Dalam
Bidang Peternakan
SAPI dengan
HORMON MANUSIA
Aplikasi Bioteknologi Dalam
Bidang Peternakan
BOVINE
SOMATOTROPIN
No. Teknik Pertanian Peternakan
Bungkil kedelai dan
White-diarrhea babi
jagung
Tanaman kapas
Fish fibrosis
antiserangga

Kelapa sawit transgenik Vaksin mulut sapi

1. DNA Rekombinan
Karet transgenik Vaksin kuku sapi
Jati kayu lebih kuat Vaksin anjing (rabies)
Anggrek dengan
Sapi perah
kesegaran lebih lama
Violetcarnation
Blue tongue domba
moonshadow
Mendeteksi virus MCF
pada hewan
Mendeteksi Begomo Mendeteksi virus
2. PCR
virus kholera babi

Mendeteksi virus rabies

3. Hibridoma - -

Perbanyakan jati
Perbanyakan anggrek

Kentang Russet Burbank

4. Kultur jaringan Anggrek -

Kina
Jati mas
Tembakau resisten
terhadap TMV
Kalus haploid tanaman
Domba Dolly
Ginkgobiloba
Monyet dengan sifat
Tumbuhan kinetik
sama
5. Kloning Kucing dengan sifat
Regenerasi tunas
sama

Hibridisasi sitoplasma
Kloning sapi
tanaman Git

6. Bayi tabung - -
Aplikasi Bioteknologi Dalam Bidang Medis dan
Kesehatan

Penerapan ini disebut sebagai bioteknologi

merah, diawali dengan tahap analisa atau
diagnosa suatu penyakit dan pengobatan
sebuah penyakit. Beberapa contoh
bioteknologi di bidang medis dan
kesahatan misalnya penggunaan
mikroorganisme pada antibiotik atau
vaksin, penggunaan mikroorganisme pada
hormon pada penyakit diabetes mellitus,
bayi tabung, Antibodi Monoklonal,
penggunaan sel intuk untuk pengibatan
penyakit sroke, dan terapi gen untuk
penyembuhan penyakit genetis.
 Aplikasi Bioteknologi Dalam
Bidang Kesehatan

Melalui rakayasa genetika, manusia

berhasil menyisipi bakteri Escherichia
coli dengan gen pembentuk insulin
pada manusia. Gen penghasil insulin
manusia tersebut dapat mengarahkan
HORMON sel E.coli untuk menghasilkan insulin.
INSULIN Dengan demikian bakteri ini mampu
membentuk insulin yang mirip dengan
insulin manusia.
 Aplikasi Bioteknologi Dalam Bidang
Kesehatan

Interferon merupakan sel-sel tubuh

yang mampu menghasilkan
senyawa kimia. Senyawa kimia
tersebut dapat membunuh virus.
Interferon berguna untuk melawan
INTERFERO infeksi dan meningkatkan sistem
N kekebalan tubuh. Produksi
interferon dilakukan melalui
rekayasa genet
 Aplikasi Bioteknologi Dalam Bidang
Kesehatan

Interferon merupakan sel-sel tubuh

yang mampu menghasilkan
senyawa kimia. Senyawa kimia
tersebut dapat membunuh virus.
Interferon berguna untuk melawan
ANTIBODI infeksi dan meningkatkan sistem
MONOKLON kekebalan tubuh. Produksi
AL interferon dilakukan melalui
rekayasa genet
Aplikasi Bioteknologi Dalam a.  DEGREDASI PLASTIK
Plastik tersusun atas bahan-bahan yang sulit terurai
Bidang Lingkungan oleh mikroorganisme. Namun ada spesies bakteri
tertentu yang dapat mendegradasinya
yaitu Clasdoporium resinae. Selain itu, baru-baru ini
ditemukan jenis ulat yang disebut mealworm yang
dapat memakan dan mengurai plastik berbahan
polistirena (penelitian yang dilakukan oleh Wei-Min Wu
dari Stanford University). 
b.  DEGREDASI TUMPAHAN MINYAK
Beberapa jenis mikroorganisme mampu menguraikan
minyak melalui mekanisme biosurfaktan yang dapat
mengurangi tegangan permukaan, sama dengan
prinsip kerja detergen. Contohnya jamur Cladosporium
resinae dan beberapa bakteri genus Pseudomonas.
IOREMEDIA c.  DETOKSIFIKASI LOGAM BERAT
SI Merkuri adalah logam berat yang mudah terakumulasi
pada organisme akuatik yang perairanya tercemar
limbah merkuri oleh industri. Jenis bakteri yang dapat
mereduksi merkuri  dengan mekanisme detoksifikasi
antara lain Bacillus, Pseudomonas, Corynebacterium,
Micococcus, dan Vibrio.
Aplikasi Bioteknologi Dalam
Bidang Lingkungan
a.  PENAMBAHAN KANDUNGAN N
Salah satu peranan bioteknologi dalam bidang ini yaitu
dengan memanfaatkan mikroba penambat nitrogen
(biofertilizer). Mikroba tersebut bekerja sama (terjadi
simbiosis) dengan tanaman leguminosa atau tanaman
kacang-kacangan. Salah satu contohnya adalah
bakteri Rhizobium leguminosarum.
b.  SEBAGAI BIODEKOMPOSER
Proses dekomposisi sampah-sampah organik 
memerlukan waktu yang cukup lama. Namun, dengan
Biofertilize pemberian aktivator dekomposisi yang berupa mikroba
r potensial (biodekomposer) prosenya dapat dipercepat.
Selain dapat meningkatkan kesuburan tanah,
dan biodekomposer yang mengandung mikroba potensial
Biodekomp yang juga dapat berperan sebagai musuh alami
penyakit jamur pada akar dan busuk pangkal batang.
oser Saat ini telah banyak produk-produk pasaran yang
menyediakan kultur untuk proses dekomposisi,
misalnya EM4 atau effective microorganisms.
Dampak Bioteknologi
 Dampak Positif  Bioteknologi
Dampak positif dari bioteknologi adalah dihasilkannya
produk-produk yang bermanfaat bagi peningkatan
kesejahtraan manusia.
a.  Bioteknologi pengelolahan limbah menghasilkan produk
biogas, kompos, dan lumpur aktif.
b.  Bioteknologi di bidang kedokteran dapat menghasilkan
obat-obatan, antar lain vaksin , antibiotik, antibodi monoklat,
dan interferon
c.  Bioteknologi dapat meningkatkan variasi dan hasil
pertanian melalui kultur jaringan, fiksasi nitrogen
pengendalian hama tanaman, dan pemberian hormon
tumbuhan.
d.  Bioteknologi dapat menghasilkan bahan bakar dengan
pengelolahan biommasa menjadi etanol (cair) dan metana (gas)
e.  Bioteknologi di bidang industri dapat menghasilkan
makanan dan minuman, antara lain pembuatan roti, nata
 Dampak Negatif Bioteknologi

a.  Menimbulkan penyakit pada manusia

Gen-gen yang mengkode untuk pembentukan antibiotic dapat saja mengalami
kecelakaan di dalam tubuh bakteri sehingga menyebabkan penyakit pada
manusia.
b.  Menimbulkan reaksi alergi
Timbulnya alergi yang disebabkan karena mengkomsumsi produk transgenic.
c.  Mengancam kelestarian alam
Jagung hasil rekayasa genetik dapat membunuh ulat yang tidak berbahaya.  
•  Rekayasa genetika dapat menghasilkan gluma-gluma super.  
•Tanaman rekayasa genetika dapat membahayakan burung yang
memakannya.   
•Menyebabkan kepunahan sebagian plasma nuftah asli karena yang
dikembangkan sekarang hanya produk rekayasa genetika saja.
d.  Berpotensi digunakan sebagai alat perang
Beberapa orang mungkin dengan sengaja menciptakan kombinasi gen-gen
baru untuk kepentingan perang (semacam senjata kimia dan senjata biologi).
Berikut Kegagalan dalam Teknik
Bioteknologi
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 Infographic Style

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