NAMA :

LEMBAR KERJA PESERTA DIDIK (LKPD)-03

KELAS : ANALISA KIMIA INSTRUMEN XIII KA 4
NO. ABSEN : TAHUN 2022/2023
KROMATOGRAFI CAIR KINERJA TINGGI

A. KOMPETENSI DASAR
3.25 Menerapkan prosedur analisis KCKT
3.26 Menerapkan penggunaan alat KCKT

B. TUJUAN
1. Menerapkan persiapan sampel, alat, dan bahan untuk analisa Kromatografi Cair Kinerja Tinggi
2. Menerapkan mekanisme pemisahan komponen sampel dengan Kromatografi Cair Kinerja
Tinggi
3. Mengidentifikasi komponen Kromatografi Cair Kinerja Tinggi
4. Menerapkan cara pengoperasian Kromatografi Cair Kinerja Tinggi

C. PETUNJUK
1. Pelajari video berikut dengan scan kode QR berikut:

Video 1 Video 2 Video 3

2. Pelajari juga modul KCKT yang telah dibagikan!

3. Kerjakan soal di bawah ini!
4. Peserta didik boleh berdiskusi dengan teman satu kelompok PKL
5. Tugas dikerjakan secara individu (masing-masing) dengan cara mencetak LKPD dan dikerjakan
di LKPD
6. Hasil pekerjaan di scan/foto dengan menampakkan nama siswa kemudian dikumpulkan di
kelas google (google classroom)
7. Waktu pengerjaan LKPD adalah 2 minggu

D. SOAL DAN HASIL PEKERJAAN

KD 3.22 Menerapkan prosedur analisis KCKT
1. Prinsip kerja HPLC/KCKT
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2. Perbedaan kolom utama dan kolom pengaman:
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3. Perbedaan fasa normal dan fasa terbalik:
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4. Syarat fasa diam untuk KCKT:
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5. Rangkaian instrumentasi HPLC/KCKT:
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6. Perbedaan elusi gradien dan elusi isokratik:
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7. Mekanisme pemisahan komponen dengan KCKT:
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KD 3.23 Menerapkan penggunaan alat KCKT

1. Komponen-komponen dalam KCKT beserta fungsinya
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2. Cara memasukkan sampel yang benar ke dalam port injeksi agar tidak mempengaruhi hasil
analisa:
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3. Tahapan pengoperasian KCKT:
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