Oleh :
PENDAHULUAN
Rate
Bab 2
Hasil dan Pemabahasan
Name: Selvy Hartati
Exercise 1: Cell Transport Mechanisms and Permeability: Activity 1: Simulating Dialysis (Simple
Diffusion) Lab Report
03/20/18 page 1
Experiment Results
Predict Question:
Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through
the 20 MWCO membrane?
Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino
acids, and the average molecular weight of a single amino acid is 135 g/mole.
Which of the following will be able to diffuse through the 200 MWCO membrane?
You correctly answered: c. the membrane pore size was too small.
Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular
weight of a single amino acid is 135 g/mole. There is no reason to run these solutes at the 20
MWCO because You correctly answered: d. glucose and albumin are both too large to pass.
Right Solute
Solute MWCO Left Solute Concentration Concentration Average Diffusion
Rate
You correctly answered: b. is slower than that for sodium because urea is a larger molecule.
Experiment Data:
03/20/18 page 3
1. The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left
beaker was to You correctly answered: b. increase the rate of diffusion.
2. Describe the difference between the rate of diffusion seen for sodium and urea.
You correctly answered: c. Urea diffused more slowly because it is larger than sodium.
3. Which of the following solutes did not pass through any of the membranes? You correctly
answered: b. albumin
03/20/18 page 4
1. Describe two variables that affect the rate of diffusion. Your answer:
2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How
well did the results compare with your prediction?
Your answer:
Karena molekul urea terlalu besar untuk melewati membran 20 MWCO
3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO
membrane. How well did the results compare with your prediction?
Your answer:
Glukosa dapat berdifusi melewati membran 200 MWCO, tidak seperti albumin yang tidak
berdifusi karena molekul albumin terlalu besar untuk melewati membran 200 MWCO.
4. Put the following in order from smallest to largest molecular weight: glucose, sodium chloride,
albumin, and urea.
Your answer:
Exercise 1: Cell Transport Mechanisms and Permeability: Activity 2: Simulated Facilitated Diffusion
Lab Report
Predict Question:
Predict Question 1: What effect do you think increasing the number of protein carriers will
have on the glucose transport rate?
Predict Question 2: What effect do you think adding Na+ Cl- will have on the glucose transport rate?
What happened to the glucose transport rate when the glucose concentration was
increased from 2 mM to 8 mM?
Describe the conditions that resulted in the fastest rate of glucose transport.
Experiment Data:
Post-lab Quiz Results
2. For facilitated diffusion, increasing the concentration of glucose on one side of the membrane is
the same as You correctly answered: b. making the concentration gradient steeper
3. When all of the membrane carriers are engaged, or busy, we say they are
You correctly answered: b. sodium is not required for glucose transport in the simula
Review Sheet Results
1. Explain one way in which facilitated diffusion is the same as simple diffusion and one way in
which it is different from simple diffusion.
Your answer:
Kedua dari difusi tersebut merupakan difusi pasif dari konsentrasi tinggi ke konsentrasi rendah.
difusi difasilitasi berbeda dari difusi sederhana karena difusi tersebut memerlukan protein pembawa
untuk difusi
2. The larger value obtained when more glucose carriers were present corresponds to an increase in
the rate of glucose transport. Explain why the rate increased. How well did the results compare with
your prediction?
Your answer:
ada lebih banyak cara dari glukosa, jadi laju difusi tersebut meningkat , seperti yang diperkirakan
3. Explain your prediction for the effect Na+ Cl- might have on glucose transport. In other words,
explain why you picked the choice that you did. How well did the results compare with your
prediction?
Your answer:
laju difusi glukosa tidak berubah karena natrium klorida tidak menggunakan pembawa protein
sehingga menghambat difusi glukosa
Name: M TEGAR NURACHMAN, I1011171047
Exercise 1: Cell Transport Mechanisms and Permeability: Activity 3: Simulating Osmotic Pressure
Lab Report
Predict Question:
Predict Question 1: What effect do you think increasing the Na+ Cl- concentration will have?
Predict Question 2: What do you think will be the pressure result of the current experimental
conditions?
Correct answer: c. There is no net movement of water because the solute concentrations are the same.
Experiment Data:
Start Conc.
Run Number Solute MWCO L Pres. L Start Conc. R Pres. R Rate
4. If you double the concentration of a nondiffusible solute that is on one side of a membrane, the
osmotic pressure will You correctly answered: c. double.
3. Water diffuses
4. Explain the effect that increasing the Na+ Cl- concentration had on osmotic pressure and why it has
this effect. How well did the results compare with your prediction?
Your answer:
5. Describe one way in which osmosis is similar to simple diffusion and one way in which it is
different.
Your answer:
osmosis dan difusi adalah transpor pasif dari konsentrasi tinggi ke konsentrasi rendah sedangkan
osmosis berbeda dari difusi karena osmosis adalah perpindahan zat pelarut melalui membran
selektif.
apabila terjadi peningkatan zat terlarut dan peningkatan jumlah milliosmoles, air akan menurun
karena air bergerak menuju konsentrasi tinggi zat terlarut
4. The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker
with the 200 MWCO membrane in place. Explain the results. How well did the results compare
with your prediction?
Your answer:
pada saat glukosa berdifusi dan beaker kanan ke beaker kiri sampai tercapai keseimbangan, maka
Albumin tidak bisa berdifusi melalui membran karena ukuran molekulnya besar, jadi tekanan
osmotik 153 mmHg tersebut adalah hasilnya
Name: Michael Nironsta Harefa
Exercise 1: Cell Transport Mechanisms and Permeability: Activity 4: Simulating Filtration Lab Report
3. The filtrate
Predict Question:
Predict Question 1: What effect will increasing the pore size of the filter have on the filtration rate?
Predict Question 2: What will happen if you increase the pressure above the beaker (the driving
pressure)?
The reason none of the solutes were present in the filtrate was that
You correctly answered: b. the solutes were all too large to pass through.
Run Number Solute MWCO Pressure Filter Rate Residue Start Conc. Filter Conc.
6. After filtration, substances that pass through the filter are called the filtrate,
which includes You correctly answered: d. all of the above.
6. Why was there not 100% recovery of the Na+ Cl- solute with any of the
membranes? You correctly answered: c. Some of the solute remained on the
membrane filter.
4. Explain in your own words why increasing the pore size increased the filtration rate. Use an
analogy to support your statement. How well did the results compare with your prediction?
Your answer:
pada hasil pengamatan yang dilakukan menunjukan bahwa peningkatkan ukuran lubang
memberikan lebih banyak cara untuk gerakan seperti yang saya duga. Bila ada kemacetan, makin
banyak mobil yang kita jalani yang memiliki pengaruh dari tekanan itu sendiri
5. Which solute did not appear in the filtrate using any of the membranes? Explain why.
Your answer:
bubuk arang, dikarenakan dari jumlah partikel dan keabsolutenya itu sendiri
6. Why did increasing the pressure increase the filtration rate but not the concentration of solutes?
How well did the results compare with your prediction?
Your answer:
pada saat percobaan ditemukan bahwa Karena tekanan membantu larutan bergerak melalui
membran, laju filtrasi meningkat seperti yang saya telah duga
Name: Michael Nironsta Harefa
Exercise 1: Cell Transport Mechanisms and Permeability: Activity 4: Simulating Filtration Lab Report
3. The filtrate
Predict Question:
Predict Question 1: What effect will increasing the pore size of the filter have on the filtration rate?
Predict Question 2: What will happen if you increase the pressure above the beaker (the driving
pressure)?
The reason none of the solutes were present in the filtrate was that
You correctly answered: b. the solutes were all too large to pass through.
Run Number Solute MWCO Pressure Filter Rate Residue Start Conc. Filter Conc.
8. After filtration, substances that pass through the filter are called the filtrate,
which includes You correctly answered: d. all of the above.
8. Why was there not 100% recovery of the Na+ Cl- solute with any of the
membranes? You correctly answered: c. Some of the solute remained on the
membrane filter.
6. Explain in your own words why increasing the pore size increased the filtration rate. Use an
analogy to support your statement. How well did the results compare with your prediction?
Your answer:
pada hasil pengamatan yang dilakukan menunjukan bahwa peningkatkan ukuran lubang
memberikan lebih banyak cara untuk gerakan seperti yang saya duga. Bila ada kemacetan, makin
banyak mobil yang kita jalani yang memiliki pengaruh dari tekanan itu sendiri
7. Which solute did not appear in the filtrate using any of the membranes? Explain why.
Your answer:
bubuk arang, dikarenakan dari jumlah partikel dan keabsolutenya itu sendiri
7. Why did increasing the pressure increase the filtration rate but not the concentration of solutes?
How well did the results compare with your prediction?
Your answer:
pada saat percobaan ditemukan bahwa Karena tekanan membantu larutan bergerak melalui
membran, laju filtrasi meningkat seperti yang saya telah duga
DAFTAR PUSTAKA
1. Ahmad, Cecep. 2008. Struktur Sel Tumbuhan dan Sel Hewan. Solo: Platimum.